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Probe Qualitativ ist die Probe positiv zu bewerten. Probe S. Typhi-O-Ag: negativ mit Werten von 0 bis 50, S. A neg. B neg. O H-Ag Gr.

B negativ mit Werten von 0 bis 50, S. B negativ mit Werten von 0 bis 50, Salmonellen-O-Ag. C negativ mit Werten von 0 bis Der Ringversuch gestaltete sich insgesamt unproblematisch.

Probe 32 war negativ und es liegt serologisch kein Hinweis auf eine Infektion vor. Probe 32 stammt von einem Patienten nach Therapie eines Ulcus vor ca.

Probe wegen der inhomogenen Ergebnisse sowohl der Zielwertlaboratorien wie auch des Teilnehmerkollektivs wurde diese Probe aus der Bewertung genommen und der Ringversuch lediglich anhand von Probe 31 bewertet.

Borrelien Probe 32 wurde einer Patientin mit Neuroborreliose M. Literatur: 1. Hunfeld K. Ringversuche in der Mikrobiologie. Ringversuche in der Infektionsserologie.

Korrespondenzadresse: Dipl. Euro 44, Dieses Buch erschien in der ersten Auflage Offenbar war die Resonanz so gut, dass nun die 2.

In den Vorworten zur 1. Im Einzelnen gliedert sich das Buch wie folgt: 1. Grundlagen Was sind Impfstoffe? Wie wirken Impfstoffe? Wie sicher sind Impfstoffe?

Wie wird geimpft? Welche Fehler gilt es zu vermeiden? Welche Argumente haben Impfskeptiker? Die jeweiligen Kapitel sind klar und logisch strukturiert.

Im Kapitel Empfehlungen wird auch kurz auf die Bedrohung durch biologische Waffen eingegangen. Ausstattung, Druck und Papier sind einwandfrei, der Preis ist gerechtfertigt.

ISBN X. Der Inhalt des Buches gliedert sich wie folgt: 1. Geschichte des Impfwesens S. Allgemeine Grundlagen S.

Formale Grundlagen S. Impfungen in besonderen Situationen S. Am Schluss findet sich ein relativ kurz gefasster aber ausreichender Index.

Ausstattung, Druck und Papier sind einwandfrei. Das Buch sollte bei keinem Arzt fehlen, der direkt oder indirekt mit Schutzimpfungen befasst ist.

Der Preis ist als angemessen anzusehen. Jahrgang, August , Heft 4, Seiten Mit der Einbeziehung der beiden letztgenannten Erreger bzw.

Erregergruppen wollen wir vor allem den zahlreichen Anfragen seitens humandiagnostisch orientierter mikrobiologischer Laboratorien und aktuellen infektiologischen Notwendigkeiten nachkommen.

Auch in den aktuellen Ringversuchssets befinden sich wieder einige Proben mit relativ geringer Menge folgender Zielorganismen: Chlamydia trachomatis Probe , B.

Im Rahmen der Testentwicklung bzw. Tabelle 1 zeigt dabei die Probenzusammensetzung und das erwartete Ergebnis Sollwert. Das aktuelle Set an Ringversuchsproben enthielt diesmal eine Probe mit einer etwas geringeren Menge an C.

Dabei wurden 7 der insgesamt 15 falsch-negativen Ergebnisse bei der schwach positiven Probe beobachtet. Interessanterweise schnitt auch diesmal die Gruppe der eigenentwickelten "in house" Assays im Durchschnitt etwas schlechter ab als die Gruppe der kommerziellen Testsystmeme.

Inhibitionsereignisse wurden von keinem der Teilnehmer beobachtet. Inhibitionsereignisse wurden von keinem der insgesamt 83 Teilnehmer mitgeteilt Die schwach positive Probe ausgenommen, fanden sich unter den mitgeteilten NAT-Ergebnissen 3 als "fraglich" eingestufte, 20 falsch-negative und 3 falsch-positive Ergebnisse.

Offensichtlich wurde mit dieser Erregermenge die untere Nachweisgrenze dieses Testsystems erreicht. Die niedriger konzentrierten Proben , und ; C.

RV Bordetella pertussis Das aktuelle Set an Ringversuchsproben enthielt je eine Probe mit hoher Menge an Zielorganismen , mit relativ geringerer Menge , ohne Zielorganismen , sowie eine Probe mit relativ hoher Menge an einer mit dem Zielorganismus eng verwandten Spezies Die etwas geringere Menge an B.

Kopien der Insertionssequenz ISwurden auch gelegentlich bei B. Die Inzidenz von B. Positiv 46 56 0 42 n. Probe enthielt diesmal eine Kultursuspension der Spezies Helicobacter salomonis, deren DNA mit den NAT-Testsystemen der insgesamt 15 Teilnehmer offensichtlich keine "spezifischen" Amplifikationsprodukte erzeugte.

Wie in der Beschreibung des Ringversuchs vermerkt, konnten die Teilnehmer auf freiwilliger Basis auch die vermeintliche Clarithromycin-Resistenz der untersuchten H.

Ergebnisse wurden hier von 10 der 15 Teilnehmer mitgeteilt, und diese waren auch durchwegs korrekt. Offensichtlich stellte vor allem der gezielte Nachweis des stx1-positiven E.

Obwohl sie nahezu gleich viele bzw. Positiv 38 13 2 2 n. Probe enthielt diesmal eine relativ hohe Menge an Borrelia garinii, die auch von 67 der insgesamt 72 Teilnehmer als positiv getestet wurde.

Lediglich 5 Teilnehmer berichteten hier ein falsch-negatives Ergebnis. Von den insgesamt 23 falsch-positive Ergebnissen entfielen 13 auf die Probe Treponema phagedenis und 10 auf Probe Borrelia recurrentis.

Artus, zumindest im Durchschnitt, ganz respektable Ergebnisse erzielen konnten. Positiv 10 67 13 69 n. Um im Rahmen dieses Ringversuchs die diagnostische Situation mit manch schwach-positivem klinischem Probenmaterial widerzuspiegeln, wurde auch diesmal eine der Proben mit einer sehr geringen Menge an L.

Die Konzentration der Zielorganismen in dieser Probe war somit nahezu identisch zu der schwach positiven Probe des vorhergegangenen Ringversuchs im April Bei dieser schwach-positiven Probe konnten lediglich 19 der insgesamt 38 Teilnehmer L.

RV Listeria spp. Neben der wohl prominentesten Spezies L. Auch wenn diese Spezies mit Ausnahme von L. Daher werden gelegentlich auch andere Listerienspezies in der einen oder anderen Probe dieses Ringversuchs zu finden sein.

Bei diesem Ringversuch besteht explizit die Option einer differenzierten Befundmitteilung. Die positiven Proben innerhalb des ausgesandten 4-er Sets enthalten daher typischerweise relativ geringe Mengen an entsprechenden Zielorganismen.

Deren Verwendung wurde im Zuge dieses Ringversuchs systematisch abgefragt. Auch wenn es sich diesmal "nur" um einen Probe-Ringversuch gehandelt hat, so zeigt die Auswertung der Ergebnisse bereits auf eindrucksvolle Weise die spezifischen Limitationen der unterschiedlichen Testformate und -konzepte.

Entsprechende Ergebnisse wurden von 6 der 31 teilnehmenden Laboratorien mitgeteilt - und diese waren erfreulicherweise durchwegs korrekt.

RV Chlamydia pneumoniae Dieser neue Ringversuch wurde in der aktuellen Ringversuchsrunde probeweise an 18 Teilnehmer versandt.

Die positiven Proben innerhalb des ausgesandten 4-er Sets enthalten daher typischerweise relativ geringe Mengen der entsprechenden Zielorganismen.

Deren Verwendung wurde im Zuge dieses Ringversuchs auch systematisch abgefragt. Die in den Tabellen dargestellten Zahlenwerte haben eher orientierenden Charakter.

Korrespondenzadresse: PD Dr. Euro 38, Er entwickelte ein dynamisches System mikrobiologischen Formenwandels und Polymorphismus, was er als das Naturgesetz mikrobiologischen Lebens ansah.

Er konnte somit, ausgehend von Hautkrankheiten eine Reihe von Infektionserregern identifizieren. Gemeinhin wird der Beginn der Beginn der Mikrobiologie als wissenschaftlich fundierte Wissenschaft mit der Entdeckung des Milzbranderregers durch Robert Koch angesehen, doch kam diese zweifellos epochale Leistung nicht aus dem Nichts, sondern baute auf den Forschungen einer Reihe hervorragender Wissenschaftler u.

Henle, Birch-Hirschfeld, Cohn, Schwann seit ca. Er leitete damit einen Paradigmenwandel ein, indem er die Medizin ins mikrobiologische Labor brachte.

Gradmann, der sich seit vielen Jahren mit Robert Koch befasst und hier sozusagen sein Opus magnum vorlegt.

Medizinische Bakteriologie und experimentelle Therapie ; Auf Reisen. Gedenktag der Entdeckung der Tuberkulose erstmals formuliert hat.

Ebenso erfreulich war die hohe Teilnehmerzahl Teilnehmer mit der Gesamtpunktzahl 75 erhielten ein Zertifikat. Probe Herkunft A: T.

Mit den Referenzlaboren aus Hamburg Dr. Schmidt aus Berlin. Am erfolgreichsten waren die Kliniken mit durchschnittlich 3,5 richtigen Antworten 3,2 von allen.

Tabelle 2 dokumentiert das arztgruppenspezifische Abschneiden. Dies wurde den Teilnehmern nicht angelastet und mit 25 Punkten bewertet.

Da ausreichend Biomasse gewachsen war, konnte man anhand der Direktmikroskopie, der Makromorphe und des klinischen Bildes zu diesem Ergebnis kommen.

Diesem Votum folgend, wurde T. Als weniger problematisch erwies sich die Erregeridentifikation. Ansorg aus Essen. Es handelte sich bei diesem Patienten um eine Mischinfektion, bei der wir annahmen, dass nur E.

Im Buch wurde deshalb nur E. Es hat sich gelohnt. Erfreulicherweise lag die Identifikationsrate deshalb nur unwesentlich hinter der von C.

Eine sichere Identifizierung gelang den meisten Teilnehmern anhand des typischen Pseudomyzels mit charakteristischen lateralen Blastosporen. Chlamydosporen fehlten im Unterschied zu C.

Rang Spezies Rate Jahr 1 Scopulariopsis brevicaulis 95,6 2 Microsporum gypseum 94,1 3 Microsporum canis 93,7 4 Microsporum gypseum 92,1 5 Trichophyton ajelloi 87,6 6 Trichophyton mentagrophytes 87,4 7 Candida glabrata 87,3 8 Candida albicans 86,0 9 Candida albicans 85,9 10 Trichophyton mentagrophytes 85,6 11 Trichophyton soudanense 85,3 12 Candida tropicalis 83,5 13 Aspergillus niger 82,5 14 Trichophyton tonsurans 82,0 15 Trichophyton rubrum 81,8 16 Candida parapsilosis 80,9 17 Trichophyton interdigitale 75,8 18 Candida guilliermondii 75,6 19 Epidermophyton floccosum 74,1 20 M.

Da der bestgemeinte Ringversuch ohne engagierte Teilnehmer nichts wert ist, laden wir Sie ein, auch im kommenden Jahr mitzuwirken. Neuer stellvertretender Ringversuchsleiter ist Herr Prof.

Am Anfang berichtete U. Die meisten der etwa T als Allergene bekannt waren; die Proteine konnten gentechnisch exprimiert und massenspektrometrisch charakterisiert werden Eine Rolle dieser Aspergillus-Antigene bei der Immunantwort des Menschen wird derzeit untersucht.

Sehr gegenwartsbezogen beleuchtete der Beitrag von J. Einmal erkannt, bereitete die Therapie dann keine Probleme. Danach ist C.

Seibold Berlin. Danach kann die kombinierte Behandlung mit liposomalem Amphotericin und Terbinafin wirksam sein. Fleck Mannheim an Caspofungin beobachtete.

Der additive Effekt von Flucytosin als Kombinationspartner von Triazolen wurde hervorgehoben. Pfizer und organisiert von R. Der zweite Ausbruchstamm hatte das gleiche Resistenzmuster, war aber sensibel gegen Amikacin.

Siphons der Waschbecken, Patientenbett, Thermoskanne mit vorbereitetem Tee. Diese Quellen werden als Infektionsreservoir diskutiert.

Chlamydien-Infektionen, Mykologie Termin: Sonnabend, Dezember Leitung: Prof. Auskunft: Weitere Informationen siehe www.

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